| Title |
Molecular confirmation, constituents and cytotoxicity evaluation of two medicinal Piper species used by the Manobo tribe of Agusan del Sur, Philippines |
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Authors |
Mark Lloyd G.Dapar, Cesar G.Demayo, Ulrich Meved, Sigrid Liede-Schumann, Grecebio Jonathan D.Alejandro |
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Publication date |
April 2020 |
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Journal |
Phytochemistry Letters |
| Volume |
36 |
| Pages |
24-31 |
| Publisher |
Elsevier |
| Abstract |
The Manobo tribe in the Philippines is culturally rich in ethnomedicinal practices and known to use popular plants locally named as 脙垄脗聙脗聹Lunas脙垄脗聙脗聺 (meaning 脙垄脗聙脗聹cure脙垄脗聙脗聺). One of these is 脙垄脗聙脗聹Lunas-bagon tapol脙垄脗聙脗聺 which was previously identified as Lunasia sp. (Rutaceae) based on vegetative morphology. The other species is 脙垄脗聙脗聹Lunas-buyo脙垄脗聙脗聺 which is similar in ethnomedicinal use and morphology. This broadly-based study verifies these two 脙垄脗聙脗聹Lunas脙垄脗聙脗聺-named specimens and evaluates the phytochemicals present and cytotoxic properties. The sequences of nuclear ITS, and plastid rbcL, and matK were investigated for species identification. Molecular confirmation using BLASTn nucleotide database query revealed that the two confused 脙垄脗聙脗聹Lunas脙垄脗聙脗聺-named specimens were members of Piperaceae and not Rutaceae. Phylogeny of Asian Piper using ITS sequences revealed 脙垄脗聙脗聹Lunas-bagon tapol脙垄脗聙脗聺 as Piper decumanum L. and 脙垄脗聙脗聹Lunas-buyo脙垄脗聙脗聺 as Piper aduncum L. with strong support (BS = 100 %). Both Piper species similarly showed the presence of alkaloids, flavonoids, steroids, tannins and fatty acids but the absence of cyanogenic glycosides. Also, P. decumanum has moderate amount of saponins while P. aduncum contains moderate amount of anthraquinones. A cytotoxic activity test using trypan blue exclusion method against normal lymphocytes from human blood showed low toxicity (91.9 % viable cells) for P. decumanum and mild toxicity (88.3 % viable cells) for P. aduncum when subjected to 1000 脙聨脗录g/ml of the stem ethanolic extracts. These results support the practical approach of molecular-based taxon identification and provide the biochemical and biological basis as to the constituents present and cytotoxic properties of these medicinal Piper species for future pharmacological research, conservation priorities and ecological management. |
| Index terms / Keywords |
BLASTnCytotoxicityITSLunasiaPhytochemicalsPiper aduncumPiper decumanum |
| DOI |
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| URL |
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Mindanao State
University - Iligan Institute of Technology