| Title |
Anti-PirA-like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease |
|
Authors |
Pedrosa-Gerasmio, Ivane R, Nakamura, Rika; Alenton, Rod Russel R; Kondo, Hidehiro; Hirono, Ikuo |
|
Publication date |
2019/05/21 |
|
Journal |
Journal of Fish Diseases |
| Volume |
42 |
| Issue |
8 |
| Pages |
Pages 1125-1132 |
| Publisher |
Wiley |
| Abstract |
Acute hepatopancreatic necrosis disease (AHPND), caused by a toxin脙垄脗聙脗聬producing Vibrio parahaemolyticus strain, has become a serious threat to shrimp aquaculture. The need to regulate antibiotic use prompted the development of alternative ways to treat infections in aquaculture including the use of chicken egg yolk immunoglobulin (IgY) for passive immunization. This study evaluated the protective effect of IgY against AHPND infection in Litopenaeus vannamei (Boone). IgY was isolated from eggs laid by hens immunized with recombinant PirA脙垄脗聙脗聬like (rPirA) and PirB脙垄脗聙脗聬like (rPirB) toxins. Whole脙垄脗聙脗聬egg powders having IgY specific to rPirA (anti脙垄脗聙脗聬PirA脙垄脗聙脗聬IgY) and rPirB (anti脙垄脗聙脗聬PirB脙垄脗聙脗聬IgY) and IgY from non脙垄脗聙脗聬immunized hen (control脙垄脗聙脗聬IgY) were mixed with basal diets at 20% concentrations and used to prefeed shrimp 3 days before the bacterial challenge test. Survival rates of the challenged shrimp fed the anti脙垄脗聙脗聬PirA脙垄脗聙脗聬IgY, anti脙垄脗聙脗聬PirB脙垄脗聙脗聬IgY and control脙垄脗聙脗聬IgY diets were 86%, 14% and 0%, respectively. Only the feed containing anti脙垄脗聙脗聬PirA脙垄脗聙脗聬IgY protected shrimp against AHPND. Increasing the concentration of rPirA antigen to immunize hens and lowering the amount of egg powder in feeds to 10% consistently showed higher survival rates in shrimp fed with anti脙垄脗聙脗聬PirA脙垄脗聙脗聬IgY (87%) compared with the control (12%). These results confirm that addition of anti脙垄脗聙脗聬PirA脙垄脗聙脗聬IgY in feeds could be an effective prophylactic method against AHPND infection in shrimp. |
| Index terms / Keywords |
acute hepatopancreatic necrosis disease, egg yolk immunoglobulin, Litopenaeus vannamei, Vibrio parahaemolyticus |
| DOI |
|
| URL |
|
Mindanao State
University - Iligan Institute of Technology