| Title |
Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function |
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Authors |
Charlotte Heymans, Catherine Spourquet, Oph脙聝脗漏lie Delcorte, Mylah Villacorte-Tabelin, S脙聝脗漏bastien Dupasquier, Younes Achouri, Siam Mahibullah, Pascale Lemoine, Maria S. Balda, Karl Matter, Christophe E. Pierreux |
|
Publication date |
2021/07 |
|
Journal |
Scientific Reports |
| Volume |
11 |
| Pages |
14519 |
| Publisher |
Nature Research |
| Abstract |
Tight junction complexes are involved in the establishment and maintenance of cell polarity and the regulation of signalling pathways, controlling biological processes such as cell differentiation and cell proliferation. MarvelD3 is a tight junction protein expressed in adult epithelial and endothelial cells. In Xenopus laevis, MarvelD3 morphants present differentiation defects of several ectodermal derivatives. In vitro experiments further revealed that MarvelD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behaviour and survival. In this work, we found that MarvelD3 is expressed from early developmental stages in the exocrine and endocrine compartments of the pancreas, as well as in endothelial cells of this organ. We thoroughly characterized MarvelD3 expression pattern in developing pancreas and evaluated its function by genetic ablation. Surprisingly, inactivation of MarvelD3 in mice did not alter development and differentiation of the pancreatic tissue. Moreover, tight junction formation and organization, cell polarization, and activity of the JNK-pathway were not impacted by the deletion of MarvelD3. |
| Index terms / Keywords |
MarvelD3, tight junction, pancreas, polarity, TAMP |
| DOI |
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| URL |
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Mindanao State
University - Iligan Institute of Technology